We have constructed a zebrafish genetic linkage map consisting of 705 simple
sequence-length polymorphism markers (SSLPs), using 44 F2 progeny from the AB x IN
reference cross (see above). The map covers 2350 centimorgans (cM) of the zebrafish
genome with an average resolution of 3.3 cM. It is a complete map in genetic mapping
terms (there is one linkage group for each of the 25 chromosomes), and it has been
confirmed by somatic-cell hybrids and centromere mapping using half-tetrad analysis.
The markers are highly polymorphic in the zebrafish strains used for genetic crossees
and provide a means to compare genetic segregation of developmental mutations between
laboratories. These markers will provide an initial infrastructure for the positional
cloning of the nearly 600 zebrafish genes identified as crucial to vertebrate development,
and will become an important anchor for the physical map of the zebrafish genome.
Markers from the original reference cross release of data are included in the figures at
the above link. For details on map construction, please refer to the manuscript cited above.
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Map Data and Graphics